Insertion junction: LMJ.RY0402.113519_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.113519
Chromosome:chromosome 8
Location:645980
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g360001 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCAAAGGAGGAAACTTAAAGTCATGGCG
Internal bar code:AGTCGTGGTGCTTCCTCTCGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:137
LEAP-Seq percent confirming:99.5699
LEAP-Seq n confirming:926
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GTGGTCAACCAGTTCGAGGT
Suggested primer 2:GTGAAGGTGAGGGTGGGTAG