Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.113525 |
Chromosome: | chromosome_3 |
Location: | 4792253 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g178500 | RIB43,RIB43A | Flagellar protofilament ribbon protein | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CGCGACGAGTGAGTGGAGCCCCAACTAGTC |
Internal bar code: | GAAGGGACGAAGGGGTGGGGGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 913 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 1282 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCGTATCATTCGGTGTGCAT |
Suggested primer 2: | CCTCTCCGTCAAACTTCTGC |