| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.113535 |
| Chromosome: | chromosome 12 |
| Location: | 2600321 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g505100 | (1 of 1) K13281 - UV DNA damage endonuclease (uvsE, UVE1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTACCAGTCCACACGCCCACGCGTCACC |
| Internal bar code: | TAACGCGAGGGCGCCCTTGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 734 |
| LEAP-Seq percent confirming: | 99.8767 |
| LEAP-Seq n confirming: | 810 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACAATTGAAGGGACAGCCC |
| Suggested primer 2: | GAGAATGCATTGGTAGGGGA |