| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.113559 |
| Chromosome: | chromosome 17 |
| Location: | 3997047 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g728864 | OPR105 | (1 of 781) IPR000104 - Antifreeze protein, type I; OctotricoPeptide Repeat protein 105 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGTCCTGCGCGCTCGTGCTTCGCCCCGCA |
| Internal bar code: | GTGAGCGGCCGCAACGGTAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 632 |
| LEAP-Seq percent confirming: | 99.3151 |
| LEAP-Seq n confirming: | 2175 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCTCGGATTATGTCTTGT |
| Suggested primer 2: | AGGCTTCTGAAGAGCAGCAG |