Insertion junction: LMJ.RY0402.113567_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.113567
Chromosome:chromosome 3
Location:557806
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g145407 (1 of 2) IPR000571//IPR002110//IPR020683 - Zinc finger, CCCH-type // Ankyrin repeat // Ankyrin repeat-containing domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGAACAAATCAACGAATGAAAGCAATGGAA
Internal bar code:TAGTATGCTATTGCGAACAAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:963
LEAP-Seq percent confirming:99.8477
LEAP-Seq n confirming:9837
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:41

Suggested primers for confirmation by PCR

Suggested primer 1:ACCGGAACTCACAGATAGCC
Suggested primer 2:CAATGGAGCTCTGGATGGAT