Insertion junction: LMJ.RY0402.113582_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.113582
Chromosome:chromosome 1
Location:2837640
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g017100 (1 of 1) IPR002123//IPR029058 - Phospholipid/glycerol acyltransferase // Alpha/Beta hydrolase fold CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACAAAGTCCCCGACGCCAACATCTGTGAC
Internal bar code:GGGCATGAGGAGGGTAGGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:636
LEAP-Seq percent confirming:99.7199
LEAP-Seq n confirming:1780
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:ACACACACACACACACACGG
Suggested primer 2:AATGTAGCGGTTGGTTTTGC