| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.113587 |
| Chromosome: | chromosome 9 |
| Location: | 2256977 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392650 | MOT39 | Predicted protein; (1 of 1) PTHR14991//PTHR14991:SF0 - FAMILY NOT NAMED // RING FINGER PROTEIN 32 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAAAGTGAGTAAATCGGGAAAGGGTCAGC |
| Internal bar code: | GCCATGAGGGGTTACAGGCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 555 |
| LEAP-Seq percent confirming: | 99.4917 |
| LEAP-Seq n confirming: | 3132 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGGATTGTGGCAGTGTCT |
| Suggested primer 2: | GAGGAGAGAGGAGGAGCGTT |