Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.113600 |
Chromosome: | chromosome_16 |
Location: | 4480770 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g667450 | TLP2 | Tubby-like protein | antisense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTATTGTCTTGCTCTAATGGTGCTGTAGGA |
Internal bar code: | ATGGTTGCCTCGTGCCACTCGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 796 |
LEAP-Seq percent confirming: | 99.4485 |
LEAP-Seq n confirming: | 1623 |
LEAP-Seq n nonconfirming: | 9 |
LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGTGGAGACCAAGTTGTCGT |
Suggested primer 2: | GAGGTAAGGCCAGGGGTAAG |