Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.113600 |
Chromosome: | chromosome_16 |
Location: | 4480780 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g667450 | TLP2 | Tubby-like protein | antisense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GAAGATTCGGCGACCCGCGGCGTGTACGAC |
Internal bar code: | AAGCCCCCTCGCCTAGCCTTGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 475 |
LEAP-Seq percent confirming: | 99.1557 |
LEAP-Seq n confirming: | 1057 |
LEAP-Seq n nonconfirming: | 9 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGTGGAGACCAAGTTGTCGT |
Suggested primer 2: | GAGGTAAGGCCAGGGGTAAG |