Insertion junction: LMJ.RY0402.113637_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.113637
Chromosome:chromosome 3
Location:2457451
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre03.g159350 (1 of 1) IPR001680//IPR011044//IPR011047//IPR015943//IPR017986//IPR027417 - WD40 repeat // Quinoprotein amine dehydrogenase, beta chain-like // Quinonprotein alcohol dehydrogenase-like superfamily // WD40/YVTN repeat-like-containing domain // WD40-repeat-containing domain // P-loop containing nucleoside triphosphate hydrolase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCAAATGGATGATGCACGACGCACGGCA
Internal bar code:TCAAACACAGGGGTCGGCGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:800
LEAP-Seq percent confirming:99.8866
LEAP-Seq n confirming:1762
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:TAACGTGCAAGAAGCAGGTG
Suggested primer 2:TACGGGGAGGTCAGTTTGTC