| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.113642 |
| Chromosome: | chromosome 12 |
| Location: | 4590836 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g522550 | FLA8,KIN2-1 | Kinesin II Motor Protein; (1 of 2) K10394 - kinesin family member 3/17 (KIF3_17) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCACCGCACCTTCTGCACTTCCTCCGGC |
| Internal bar code: | AGACTGTACTGCGTCCCGGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 788 |
| LEAP-Seq percent confirming: | 81.7944 |
| LEAP-Seq n confirming: | 939 |
| LEAP-Seq n nonconfirming: | 209 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGGTAACAGCCCACAACA |
| Suggested primer 2: | CACCTCCTGAGCTTACAGCC |