Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.113646 |
Chromosome: | chromosome_3 |
Location: | 5005435 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g180450 | FAP215,FPN1 | 5\'-nucleotidase and Flagellar Associated Protein | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCACCGTAGTGAACCGGAGTTGAGGATGGC |
Internal bar code: | GAGCTACCTTCTGGTCGGTACG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 166 |
LEAP-Seq percent confirming: | 99.7033 |
LEAP-Seq n confirming: | 336 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTCAACGTGTTGGTGTTTGG |
Suggested primer 2: | GACCGCCACCTTGTACTTGT |