Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.113666 |
Chromosome: | chromosome_12 |
Location: | 129155 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g484250 | DHC1,PF9,ROC34,IDA1 | Flagellar inner arm dynein 1 heavy chain alpha (I1/f alpha) | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTTCTCAAACAGGAACGGGAAGCCGTACTG |
Internal bar code: | CGGATTCAAAAAAAACAAACGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 136 |
LEAP-Seq percent confirming: | 99.8331 |
LEAP-Seq n confirming: | 598 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATGACGAAGAACAGGATGGC |
Suggested primer 2: | CAGTTTGAGGAGAAGACGGC |