Insertion junction: LMJ.RY0402.113666_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.113666
Chromosome:chromosome 12
Location:129155
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g484250 DHC1,IA1-DHC1alpha,IDA1,ROC34,PF9 (1 of 1) PTHR10676//PTHR10676:SF135 - DYNEIN HEAVY CHAIN FAMILY PROTEIN // DYNEIN HEAVY CHAIN 10, AXONEMAL; Inner Arm Dynein Heavy Chain 1/f-alpha CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGACGAGTACATTGACCCCGTCATTGAC
Internal bar code:TTGCGGTTCTTGGCTAATAACT

Confirmation - LEAP-Seq

LEAP-Seq distance:116
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:284
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ATGACGAAGAACAGGATGGC
Suggested primer 2:CAGTTTGAGGAGAAGACGGC