| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.113685 |
| Chromosome: | chromosome 17 |
| Location: | 1532498 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g707250 | MFT29 | Major facilitator superfamily transporter; (1 of 82) IPR020846 - Major facilitator superfamily domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTCCCCACTATGCCTGCCACGTTGCCAA |
| Internal bar code: | TGCCGACAGCTCGGGCGGGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 396 |
| LEAP-Seq percent confirming: | 99.8136 |
| LEAP-Seq n confirming: | 2677 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTGTAAACTCCAGCCCAA |
| Suggested primer 2: | TTGGTCTAGTGGCAGAGGCT |