| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.113686 |
| Chromosome: | chromosome 2 |
| Location: | 5725633 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g110200 | THO1 | Oligoendopeptidase; (1 of 3) 3.4.24.59 - Mitochondrial intermediate peptidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGGGTGTTGGTACACGGCACCCCACGAC |
| Internal bar code: | GTATTTGTTGACCGCGTTGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 422 |
| LEAP-Seq percent confirming: | 99.6641 |
| LEAP-Seq n confirming: | 890 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTTTGATGCTGTTTTTCA |
| Suggested primer 2: | ATTCGTGTTGCGGACTTACC |