Insertion junction: LMJ.RY0402.113712_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.113712
Chromosome:chromosome 17
Location:6016611
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g740800 (1 of 5) PF06742//PF06863 - Protein of unknown function (DUF1214) (DUF1214) // Protein of unknown function (DUF1254) (DUF1254) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGAGCTAAAACCGGGTCAGGCCGCGAGG
Internal bar code:TTTGCCAATTGACGCCTTCTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:886
LEAP-Seq percent confirming:99.6829
LEAP-Seq n confirming:943
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTCAGGGTCAGCTAGGTT
Suggested primer 2:CAGGATTTCGAAGGGAATGA