| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.113735 |
| Chromosome: | chromosome 13 |
| Location: | 5041146 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g606974 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTAGTCACAGTCACGTGCACGGGGGATT |
| Internal bar code: | CCAACAAGTAGTTCGCTGTCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1002 |
| LEAP-Seq percent confirming: | 98.0538 |
| LEAP-Seq n confirming: | 1058 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATGCTGCTCTGGTGCGTA |
| Suggested primer 2: | CAACTGTGAGGATGTGTGGG |