| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.113739 |
| Chromosome: | chromosome 3 |
| Location: | 7540918 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g205250 | ELG4 | Exostosin-like glycosyltransferase 4; (1 of 39) PTHR11062//PTHR11062:SF21 - EXOSTOSIN HEPARAN SULFATE GLYCOSYLTRANSFERASE -RELATED // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTCCGCACGACAGCTGGCTGGCCCCCTC |
| Internal bar code: | TTGCAACGACCGTGCTCCAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 259 |
| LEAP-Seq percent confirming: | 99.7 |
| LEAP-Seq n confirming: | 997 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGCGAAGTTGTAGCCAGG |
| Suggested primer 2: | GGAACAACTCCTCATCCAGC |