| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.113748 |
| Chromosome: | chromosome 9 |
| Location: | 2821238 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388800 | GDH1 | Glutamate dehydrogenase; (1 of 2) 1.4.1.3 - Glutamate dehydrogenase (NAD(P)(+)) / Glutamic dehydrogenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGATGCGAGGGCGAGAGCACGGGCGGGAG |
| Internal bar code: | TGTGCCGTTAAGGGTCTGAAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 445 |
| LEAP-Seq percent confirming: | 99.717 |
| LEAP-Seq n confirming: | 5286 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCCGTATTGACGTTGTTG |
| Suggested primer 2: | TACGACAAACGAGTCAAGCG |