| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.113749 |
| Chromosome: | chromosome 3 |
| Location: | 111981 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g143987 | (1 of 1) IPR011016//IPR014048 - Zinc finger, RING-CH-type // Methylated-DNA-[protein]-cysteine S-methyltransferase, DNA binding | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGCGCCCCCGTTGCCTTCCAGTCCATAA |
| Internal bar code: | TCAGCGGGATAGCGGGCTCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 300 |
| LEAP-Seq percent confirming: | 98.2791 |
| LEAP-Seq n confirming: | 5768 |
| LEAP-Seq n nonconfirming: | 101 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTCCTGTGGTGTTCAGGG |
| Suggested primer 2: | CGCCTAAACGTATCGTGGAT |