Insertion junction: LMJ.RY0402.113749_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.113749
Chromosome:chromosome 3
Location:111991
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g143987 (1 of 1) IPR011016//IPR014048 - Zinc finger, RING-CH-type // Methylated-DNA-[protein]-cysteine S-methyltransferase, DNA binding 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGACCCGTACGACAGCGCACAGGTGGTGC
Internal bar code:GGTCCAAATACGGTCCACCGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:908
LEAP-Seq percent confirming:98.5842
LEAP-Seq n confirming:2646
LEAP-Seq n nonconfirming:38
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:AGTTCCTGTGGTGTTCAGGG
Suggested primer 2:CGCCTAAACGTATCGTGGAT