Insertion junction: LMJ.RY0402.113767_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.113767
Chromosome:chromosome 8
Location:4240824
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre08.g380650 GT90-13,GT90F13 GT90 family protein 13; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTTGTCTTGTGTCTATGTACACGCACACG
Internal bar code:CTGCCAACGTGGGCGCGTGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:85
LEAP-Seq percent confirming:99.8238
LEAP-Seq n confirming:1133
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CGAATGAAGGACAGGGTGTT
Suggested primer 2:ACCACCACCTACACACCCAT