| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.113771 |
| Chromosome: | chromosome_14 |
| Location: | 770363 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g612950 | CYG17 | Adenylate/guanylate cyclase | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGCATGTCTGGCGGGGCGCGCTACGGCGCC |
| Internal bar code: | CGTCCGCGGGTGATGTCACTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 195 |
| LEAP-Seq percent confirming: | 50.2415 |
| LEAP-Seq n confirming: | 1040 |
| LEAP-Seq n nonconfirming: | 1030 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGTCACCACACATTGACC |
| Suggested primer 2: | CTCTCCACCTCGGGTGACT |