| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.113817 |
| Chromosome: | chromosome 4 |
| Location: | 2187651 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g219851 | (1 of 2) PF11360 - Protein of unknown function (DUF3110) (DUF3110) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTATGCGCCGCAGCCAAGTCGAAAGGTG |
| Internal bar code: | GATAACACGATAGCGTTCCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 745 |
| LEAP-Seq percent confirming: | 99.8867 |
| LEAP-Seq n confirming: | 5292 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTAAACCCTGGATGTGCG |
| Suggested primer 2: | CGTTCCAGGCTTGTCTGTCT |