| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.113851 |
| Chromosome: | chromosome 17 |
| Location: | 3386889 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g723900 | FAP131,ROC103 | Flagellar Associated Protein 131 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGCACGGAGGTCAAACAAGTGCTCCGCC |
| Internal bar code: | GTAGCCCGTCAGCTTACTCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1031 |
| LEAP-Seq percent confirming: | 99.68 |
| LEAP-Seq n confirming: | 7475 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGCAGGCACGCTTAGATA |
| Suggested primer 2: | TAAGCATCATCGCTCACTGG |