Insertion junction: LMJ.RY0402.113859_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.113859
Chromosome:chromosome 16
Location:632099
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre16.g692000 (1 of 9) 3.1.3.2 - Acid phosphatase / Phosphomonoesterase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCAGATACACGATTTACGTGATCTGGCAGA
Internal bar code:AATCGTAACTGGACGCCCCTGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:833
LEAP-Seq percent confirming:47.4209
LEAP-Seq n confirming:2188
LEAP-Seq n nonconfirming:2426
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:CCACACCTCCAGGAACAGAT
Suggested primer 2:CCAGCCCAAACTAAACCAAA