| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.113874 |
| Chromosome: | chromosome 17 |
| Location: | 3929383 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g728450 | (1 of 1) IPR006626//IPR010308//IPR011050 - Parallel beta-helix repeat // TRP-like family // Pectin lyase fold/virulence factor | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTGACCATCCAGCCCAACGTGTCCGTCA |
| Internal bar code: | GACTAGTGTCGTCCATACGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 930 |
| LEAP-Seq percent confirming: | 99.0688 |
| LEAP-Seq n confirming: | 3298 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTATGGTCATGGCTGACG |
| Suggested primer 2: | AGGCGCTAATGAGAGCTGAG |