| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.113897 |
| Chromosome: | chromosome 12 |
| Location: | 8343749 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g549450 | (1 of 1) 3.7.1.5 - Acylpyruvate hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTTAGGCCCCTCTGGTACACAACTGATC |
| Internal bar code: | ATCTCGGCGCTATACTGGCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 57 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 78 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCTAGAAGACGCCGACAG |
| Suggested primer 2: | GTCCAAGCTCAAGTCCAAGC |