| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.113953 |
| Chromosome: | chromosome 13 |
| Location: | 1447281 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g572150 | NAT29 | N-acetyltransferase; (1 of 1) PTHR14744:SF15 - N-ALPHA-ACETYLTRANSFERASE 60 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATTCTGCCGCAGCGCCCTGAGCGCGAACC |
| Internal bar code: | TGTCTGGTGCCCTTCCCGGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 718 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 686 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGGTCATGTGAGGTCCGT |
| Suggested primer 2: | TTACGAGCGGAAAGACTCGT |