| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.113957 |
| Chromosome: | chromosome 5 |
| Location: | 1817844 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g242100 | SUB8 | (1 of 1) PTHR10795//PTHR10795:SF157//PTHR10795:SF371 - PROPROTEIN CONVERTASE SUBTILISIN/KEXIN // MEMBRANE-BOUND TRANSCRIPTION FACTOR PEPTIDASE, SITE 1 // SUBFAMILY NOT NAMED; Subtilisin-like protease | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGACATGGACGTGCCGCTCCGAGCCGCCA |
| Internal bar code: | GCTCGCTTCTTCCGGTGTAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 185 |
| LEAP-Seq percent confirming: | 27.3833 |
| LEAP-Seq n confirming: | 991 |
| LEAP-Seq n nonconfirming: | 2628 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTTGGAGGTGAGGTGAGG |
| Suggested primer 2: | CCAGCCAGGGTATCAACAGT |