Insertion junction: LMJ.RY0402.113983_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.113983
Chromosome:chromosome 13
Location:1051601
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g568700 (1 of 1) K13123 - G patch domain-containing protein 1 (GPATCH1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGTGGGGGTGGGTTGTAGATAACAGCCCA
Internal bar code:CGCACTTTCGTTTCGCGGTGTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:339
LEAP-Seq percent confirming:99.5792
LEAP-Seq n confirming:9702
LEAP-Seq n nonconfirming:41
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CCCGCACATCCTCTACCTAA
Suggested primer 2:GATAGGGAGCACAAGGGACA