Insertion junction: LMJ.RY0402.113995_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.113995
Chromosome:chromosome 5
Location:3169237
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre05.g239500 FAP38 (1 of 1) IPR000104//IPR009068 - Antifreeze protein, type I // S15/NS1, RNA-binding; Flagellar Associated Protein 38 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAAGGATCGGAGTTGTAGCCACGCACATTG
Internal bar code:CCCCGGAAGTATGTGGGGCGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:344
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1884
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTCCATTTGCTTGATCTG
Suggested primer 2:CCTCCCAGTCCCACTCAATA