Insertion junction: LMJ.RY0402.113999_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.113999
Chromosome:chromosome 1
Location:4600818
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g031550 CSB6 (1 of 12) IPR004843//IPR029052 - Calcineurin-like phosphoesterase domain, apaH type // Metallo-dependent phosphatase-like; Probable transposon-derived protein of Chlamydomonas-Specific family B intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCGCAGCACCGTGGGCGCTGTCCACACG
Internal bar code:GCTGTCCGCTGTGTGTTTTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:518
LEAP-Seq percent confirming:99.7642
LEAP-Seq n confirming:3808
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TCATGCGAACGGTGTGTATT
Suggested primer 2:CCTACTCTCCCTGCCTCCTT