Insertion junction: LMJ.RY0402.114003_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.114003
Chromosome:chromosome 6
Location:8129700
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g304950 (1 of 100) IPR001810 - F-box domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGACTAGAACCAAAACGTGCGCAGCATCCC
Internal bar code:GCCAGTATTTCTGTTTCTATTA

Confirmation - LEAP-Seq

LEAP-Seq distance:912
LEAP-Seq percent confirming:98.9757
LEAP-Seq n confirming:3865
LEAP-Seq n nonconfirming:40
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:CGGAGTGATAGTGCTCGTCA
Suggested primer 2:TGGTCGCTCATTCTCATCTG