Insertion junction: LMJ.RY0402.114023_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.114023
Chromosome:chromosome 6
Location:1971993
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g263950 (1 of 1) 3.6.3.9 - Sodium/potassium-exchanging ATPase / Sodium/potassium-transporting ATPase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGATACTTGATATTCACTACATCCGCTTA
Internal bar code:AAAGGATGTTCGGAATTGGTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1145
LEAP-Seq percent confirming:99.3497
LEAP-Seq n confirming:4278
LEAP-Seq n nonconfirming:28
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TTATTTGCGTGTTGCATGGT
Suggested primer 2:GCTGTTTGTCGTTTGGGTTT