| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.114038 |
| Chromosome: | chromosome 10 |
| Location: | 1193967 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g426250 | (1 of 1) K14800 - pre-rRNA-processing protein TSR2 (TSR2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTTGCCCAAGCAGCCCTACCGAGTCCTC |
| Internal bar code: | GAATAGTCCGAATAGGACCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 829 |
| LEAP-Seq percent confirming: | 94.4421 |
| LEAP-Seq n confirming: | 2260 |
| LEAP-Seq n nonconfirming: | 133 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCAATTGAATAGAGGCAA |
| Suggested primer 2: | ATGCTAAAGGCTGCACTGGT |