Insertion junction: LMJ.RY0402.114061_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.114061
Chromosome:chromosome 4
Location:1211614
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g215400 AKC3 conserved expressed protein related to ABC1/COQ8 mitochondrial putative ser/thr kinase; (1 of 2) PTHR10566//PTHR10566:SF86 - CHAPERONE-ACTIVITY OF BC1 COMPLEX CABC1 -RELATED // ABC1 PROTEIN KINASE 6 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCATGACACAGGTGTGCGGCTGTGAGCCC
Internal bar code:CGTCTAGGCGCCCTTTGGGGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:494
LEAP-Seq percent confirming:99.6683
LEAP-Seq n confirming:601
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AACAAGAAACCCGCAAACAC
Suggested primer 2:CTCATGTGTGTGCTGTCACG