| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.114091 |
| Chromosome: | chromosome 1 |
| Location: | 4030563 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g026250 | AGA1 | Alpha-galactosidase; (1 of 1) 3.2.1.22 - Alpha-galactosidase / Melibiase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTATGGAGGCGTGAGGAACGCCCGCATT |
| Internal bar code: | TATCCGGAGGGTCGCGCATTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 110 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCAACAGCGACCTATCTG |
| Suggested primer 2: | AGTATCACCGCCATGGCTAC |