| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.114124 |
| Chromosome: | chromosome 16 |
| Location: | 843613 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g647800 | (1 of 1) IPR000104//IPR001932//IPR015655 - Antifreeze protein, type I // PPM-type phosphatase domain // Protein phosphatase 2C | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTTAGGGCAGCTGGTTTCGCTTTTCCAG |
| Internal bar code: | CGCGCATGCTGGGGCCACCCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 924 |
| LEAP-Seq percent confirming: | 99.8662 |
| LEAP-Seq n confirming: | 3731 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGCCTATTAGCTCCCAAG |
| Suggested primer 2: | CAACCACCATCAAGCACAAC |