Insertion junction: LMJ.RY0402.114127_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.114127
Chromosome:chromosome 4
Location:2864784
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g224400 (1 of 1) 3.6.3.25//3.6.3.44 - Sulfate-transporting ATPase // Xenobiotic-transporting ATPase / Steroid-transporting ATPase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGTAACTTCGATGCCCAGCAACCCGTATC
Internal bar code:GGTTGGTGGAAGGGGATAAGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:926
LEAP-Seq percent confirming:99.9025
LEAP-Seq n confirming:2049
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCTAGCTTGTCCGTTTCG
Suggested primer 2:AGTCGCATACCACCACTTCC