| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.114135 |
| Chromosome: | chromosome 17 |
| Location: | 1669163 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g708400 | SUB12 | (1 of 1) 3.4.21.66 - Thermitase / Thermophilic Streptomyces serine protease; Subtilisin-like protease | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCCGCCTTTACGTCCACCCTCGTCCTTG |
| Internal bar code: | CTCGTTGCCGAAGGTTCAGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 115 |
| LEAP-Seq percent confirming: | 92.8571 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAATGAGCTGACGGATCTGA |
| Suggested primer 2: | GATTAAGCAGTGTGCAGCCA |