| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.114147 |
| Chromosome: | chromosome 16 |
| Location: | 2524202 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g661150 | CGL5 | Conserved in the Green Lineage; (1 of 2) 5.2.1.14 - Beta-carotene isomerase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGAAGGGTTCTGAGGATGCACGGACAAT |
| Internal bar code: | TGTTCTAGCCCTGTTCCGTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 529 |
| LEAP-Seq percent confirming: | 99.6548 |
| LEAP-Seq n confirming: | 6929 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGCTGTAATCTTAGCGAC |
| Suggested primer 2: | AGGGAGAAGATCCTTGGGAA |