Insertion junction: LMJ.RY0402.114159_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.114159
Chromosome:chromosome 11
Location:2188868
Confidence (%):73
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGTGTGTCAGTGTGTTTGCAGAGGAGACA
Internal bar code:GATCGTTGTACGAAAACGCGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:162
LEAP-Seq percent confirming:99.5798
LEAP-Seq n confirming:2133
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TCCTCTACGCGGTGTCTTCT
Suggested primer 2:CCGCAAACACCCATTACTCT