Insertion junction: LMJ.RY0402.114159_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.114159
Chromosome:chromosome 17
Location:2701353
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g717950 PHC28,PHC6 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 6 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTCGAGACCCGCGAACCATGCGGCTCACC
Internal bar code:GCAGCACTCAGCGTTCGCGCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:331
LEAP-Seq percent confirming:99.7094
LEAP-Seq n confirming:2402
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CTGGAAGAGCTTCAATTGCC
Suggested primer 2:CTCCTGGTAACCCCTCAACA