| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.114172 |
| Chromosome: | chromosome 6 |
| Location: | 3075098 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g274600 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCCATGCAGGCATGCGTCATCAACCTCC |
| Internal bar code: | CAAGCAACCACGCACACGGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 206 |
| LEAP-Seq percent confirming: | 99.4165 |
| LEAP-Seq n confirming: | 2215 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATGTGGGGATTTCCATAG |
| Suggested primer 2: | GAACTGCAGGGGTAGTCGTC |