| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.114210 |
| Chromosome: | chromosome 16 |
| Location: | 1691325 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g654700 | PHC61 | Pherophorin-chlamydomonas homolog 61; (1 of 71) PF12499 - Pherophorin (DUF3707) | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGTGGGGGCACGGCGGGCGGGCACGGCAA |
| Internal bar code: | GCTCGGTGAACCACTTGTGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 664 |
| LEAP-Seq percent confirming: | 99.7749 |
| LEAP-Seq n confirming: | 1773 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAAACTACCCCATTGCACG |
| Suggested primer 2: | TACCCGTTTCCTTACAACGC |