Insertion junction: LMJ.RY0402.114250_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.114250
Chromosome:chromosome 5
Location:2537051
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g236000 (1 of 2) PTHR23257//PTHR23257:SF446 - SERINE-THREONINE PROTEIN KINASE // LIM DOMAIN-CONTAINING SERINE/THREONINE-PROTEIN KINASE DDB_G0286997-RELATED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCATCCCCGCCTGCTGCCTACTTCGCTGCG
Internal bar code:TGTCTCACTCGCATTGCGTCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:344
LEAP-Seq percent confirming:99.3789
LEAP-Seq n confirming:480
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TTGGTTCGGTCTAGTTTGGG
Suggested primer 2:ATTCCCAAAGTCCAATTCCC