Insertion junction: LMJ.RY0402.114255_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.114255
Chromosome:chromosome 13
Location:4147889
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g591851 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCGCAGACACATCTTGGTCCTCGATCGAC
Internal bar code:GTTCCTCTCTCGGTTTAGTAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:97.381
LEAP-Seq n confirming:409
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CAGGGAACGTCAAGAATGGT
Suggested primer 2:ACATCATGTCGTGCGTGATT