| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.114303 |
| Chromosome: | chromosome 7 |
| Location: | 4239561 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g341925 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCCTCGGCGCTGTCGAGGTTAGTGTGGG |
| Internal bar code: | GCCCCACCAACAGGTGTAATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 9 |
| LEAP-Seq percent confirming: | 98.6047 |
| LEAP-Seq n confirming: | 424 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCAACGACACCAGTTCAT |
| Suggested primer 2: | TGTCGGAAGGAGGAATAGGA |